Table 1 Oligonucleotides used for creation of anti-IL12B shRNA expression cassettes
From: Targeting of human interleukin-12B by small hairpin RNAs in xenografted psoriatic skin
| shRNA | Oligonucleotide sequence (target sequences are underlined) |
|---|---|
| shIL12B #1 | Sense: 5'-ctagccc cctcacctgtgacacccct actcgagaaggggtgtcacaggtgaggtttttggaaa-3' |
| Antisense: 5'-cgcgtttccaaaaacctcacctgtgacaccccttctcgagtaggggtgtcacaggtgaggggg-3' | |
| shIL12B #2 | Sense: 5'-ctagccc atgccagcattagcgtgcg actcgagacgcacgctaatgctggcattttttggaaa-3' |
| Antisense: 5'-cgcgtttccaaaaaatgccagcattagcgtgcgtctcgagtcgcacgctaatgctggcatggg-3' | |
| shIL12B #3 | Sense: 5'-ctagccc ggtcttaggctctggcaaa actcgagatttgccagagcctaagacctttttggaaa-3' |
| Antisense: 5'-cgcgtttccaaaaaggtcttaggctctggcaaatctcgagttttgccagagcctaagaccggg-3' | |
| shIL12B #4 | Sense: 5'-ctagccc ggaatttggtccactgata actcgagatatcagtggaccaaattcctttttggaaa-3' |
| Antisense: 5'-cgcgtttccaaaaaggaatttggtccactgatatctcgagttatcagtggaccaaattccggg-3' | |
| shIL12B #5 | Sense: 5'-ctagccc gcagacagatgcaaagaaa actcgagatttctttgcatctgtctgctttttggaaa-3' |
| Antisense: 5'-cgcgtttccaaaaagcagacagatgcaaagaaatctcgagttttctttgcatctgtctgcggg-3' | |
| shIL12B #6 | Sense: 5'-ctagccc gatgggaacgcaagagatact actcgagaagtatctcttgcgttcccatctttttggaaa-3' |
| Antisense: 5'-cgcgtttccaaaaagatgggaacgcaagagatacttctcgagtagtatctcttgcgttcccatcggg-3' | |
| shIL12B #7 | Sense: 5'-ctagccc gggccttcatgctatttaa actcgagattaaatagcatgaaggccctttttggaaa-3' |
| Antisense: 5'-cgcgtttccaaaaagggccttcatgctatttaatctcgagtttaaatagcatgaaggcccggg-3' |